Zinc-finger nucleases
نویسندگان
چکیده
منابع مشابه
Genome engineering with zinc-finger nucleases.
Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular DNA repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies. This article briefly reviews the history of ZFN development and summarizes applications that have been m...
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Agricultural biotechnology is limited by the inefficiency and unpredictability of conventional random mutagenesis and transgenesis. We have developed a broadly applicable, versatile alternative: the use of designed zinc-finger nucleases (ZFNs) that induce double-stranded breaks (DSB) at their target locus. We show that ZFNs designed to cleave an endogenous maize gene disrupt their target via DS...
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One advantage of the zebrafish model system is the ability to use forward genetics to reveal critical gene functions by their mutant phenotype. Reverse genetic tools are available, although it is more challenging and time-consuming to identify mutations in specific genes of interest and virtually impossible to induce mutations in a targeted manner. Two recent papers have shown that locus-specif...
متن کاملHighly active zinc-finger nucleases by extended modular assembly.
Zinc-finger nucleases (ZFNs) are important tools for genome engineering. Despite intense interest by many academic groups, the lack of robust noncommercial methods has hindered their widespread use. The modular assembly (MA) of ZFNs from publicly available one-finger archives provides a rapid method to create proteins that can recognize a very broad spectrum of DNA sequences. However, three- an...
متن کاملEfficient gene targeting in Drosophila with zinc-finger nucleases.
This report describes high-frequency germline gene targeting at two genomic loci in Drosophila melanogaster, y and ry. In the best case, nearly all induced parents produced mutant progeny; 25% of their offspring were new mutants and most of these were targeted gene replacements resulting from homologous recombination (HR) with a marked donor DNA. The procedure that generates these high frequenc...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2010
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.f.328